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Natural Crosslinker Genipin

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Natural Crosslinker Genipin

Product Abstract:

Genipin CAS 6902-77-8. Natural biochemical crosslinker which is extracted from gardenia fruit.

Product Description


SYBiochemical can produce different purities of genipin to meet your needs with our unique enzyme.If you need more,feel free to contact us.

Purity: 98%+;95%; 90%;80%;etc.

[CAS No.]?6902-77-8

[Original]: Dry fruits of Gardenia Jasminoides Ellis.

[Appearance]:White powder or crystalline.


1.It will slowly yellowing when exposure to air long term.

2.The inhibition of Gastric function was anticholinergic, it’s the strongest substances which can inhibit the mutagenic activity of the mutagen among the ever found iridoid glycosides.

3.Soluble in ethanol, acetone, chloroform, diethyl ether, etc.; slightly soluble in water.

Application: Natural crosslinker

1.Schek RM, Michalek AJ, Iatridis JC. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair. Eur Cell Mater.2011 Apr 18;21:373-83.

Abstract?Treatment of damaged intervertebral discs is a significant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fibrosus. An annular repair material should meet three specifications: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fibrin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fibrin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fibrin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fibrin alone. Finally, lap tests were performed to evaluate adhesion between fibrin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fibrin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fibrin gels show promise as a gap-filling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fibrosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.


2?Pabittei DR, Heger M, van Tuijl S, Simonet M, de Boon W, van der Wal AC, Balm R, de Mol BA. Ex vivo proof-of-concept of end-to-end scaffold-enhanced laser-assisted vascular anastomosis of porcine arteries. J Vasc Surg. 2014 Mar 7. pii: S0741-5214(14)00166-9. doi: 10.1016/j.jvs.2014.01.064. [Epub ahead of print]

PMID: 24613189

3. Fernandes M, Gonçalves IC, Nardecchia S, Amaral IF, Barbosa MA, Martins MC. Modulation of stability and mucoadhesive properties of chitosan microspheres for therapeutic gastric application. Int J Pharm. 2013 Sep 15;454(1):116-24. doi: 10.1016/j.ijpharm.2013.06.068. Epub 2013 Jul 11.

PMID: 23850814

4. Liu TX, Wang Z. Collagen crosslinking of porcine sclera using genipin. Acta Ophthalmol. 2013 Jun;91(4):e253-7. doi: 10.1111/aos.12172. Epub 2013 May 28.

PMID: 23710671

5. Nata IF, Wang SS, Wu TM, Lee CK. β-Chitin nanofibrils for self-sustaining hydrogels preparation via hydrothermal treatment. Carbohydr Polym. 2012 Nov 6;90(4):1509-14. doi: 10.1016/j.carbpol.2012.07.022. Epub 2012 Jul 16.


6. Schek RM, Michalek AJ, Iatridis JC. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair. Eur Cell Mater. 2011 Apr 18;21:373-83.


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